Certified Tissue Bank Specialist (CTBS) Practice Test

Which factor is crucial for maintaining the viability of retrieved tissue?

1. Temperature control

2. Light exposure

3. Humidity levels

4. Oxygen availability

The correct answer is: Temperature control

Maintaining the viability of retrieved tissue is primarily dependent on temperature control. Proper temperature regulation is essential because tissue can be highly sensitive to temperature fluctuations. High temperatures can lead to cellular damage, denaturation of proteins, and accelerated metabolic processes that ultimately reduce viability. Conversely, temperatures that are too low may slow metabolism but could also lead to the formation of ice crystals within cells, causing mechanical damage. In practice, tissues are typically stored and transported at specific temperatures, such as refrigeration or freezing, depending on the type of tissue and the intended use. For example, allograft tissues often require storage at cold temperatures to preserve functional integrity while preventing microbial growth. This careful management of temperature ensures that tissues remain viable for transplantation or other medical applications. Light exposure, humidity levels, and oxygen availability, while they may have some impact on certain biological materials, are not as critical as temperature control when it comes to preserving tissue viability. Most retrieved tissues are shielded from light and are not dependent on humidity levels for their short-term viability, and while oxygen levels may be important during the preservation of cells or organ cultures, for retrieved tissues, temperature is the primary factor to ensure that they remain viable until they are used.